Primary sequence of Papain- like protease of Covid-19
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Biology
Primary sequence of Papain- like protease of Covid-19
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The severe acute respiratory syndrome coronavirus papainlike protease SARSCoV PLpro is involved in the processing of the viral polyprotein and thereby contributes to the biogenesis of the virus replication complex Structural bioinformatics has revealed a relationship for the SARSCoV PLpro to herpesvirusassociated ubiquitinspecific protease HAUSP a ubiquitinspecific protease indicating potential deubiquitinating activity in addition to its function in polyprotein processing T Sulea H A Lindner E O Purisima and R Menard J Virol 7945504551 2005 In order to confirm this prediction we overexpressed and purified SARSCoV PLpro amino acids aa1507 to 1858 from Escherichia coli The purified enzyme hydrolyzed ubiquitin7amino4methylcoumarin UbAMC a general deubiquitinating enzyme substrate with a catalytic efficiency of 13100 M1s1 220fold more efficiently than the small synthetic peptide substrate ZLRGGAMC which incorporates the Cterminal four residues of ubiquitin In addition SARSCoV
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