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Problem 1. Mitochondria may be prepared from cells and tested in cell free experiments. If supplied...

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Biology

Problem 1. Mitochondria may be prepared from cells and tested incell free experiments. If supplied with pyruvate and oxygen,isolated mitochondria are capable of aerobic metabolism.

C. You as the investigator measure the energy state of themitochondria as the ratio [NADH]/[NAD+]

1. Describe how the ratio is high and low for high and lowenergy charge, respectively

2. You remove the supply of oxygen. Explain how the ratiochanges in response

3. You add an inhibitor of ATP synthase. Explain how the ratiochanges in response

D. You as the investigator makes three measurements at the endof a test that lasts 10 min (exact time is not important). Youmeasure 10 nmol (10-9 moles) of pyruvate consumed (this number isimportant). You also measure the nmol of CO2 and citrateproduced.

1. Explain how 30 nmol of CO2 were produced.

2. Explain how 0 nmol of citrate were produced.

3. Based on the measurements above, predict how many nmol ofacetyl-CoA were produced. Explain.

Answer & Explanation Solved by verified expert
4.5 Ratings (993 Votes)
1 The ratio of NADHNAD will be low for high energy charge and will be high for low energy charge In presence of oxygen and highly charged mitochondria the electron transport chain will transfer electrons from complex I to complex III to complex IV and finally O2 During this electron transfer NADH is    See Answer
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