Q1. Cell survival curves
Suppose you did an experiment to generate a cell survivalcurve.
a) Describe what steps you would take to do the experiment.
b) What is meant by plating efficiency?
c) In your experiment you irradiate cells using 6 MV photons ona linear accelerator. You collect the data shown in the tablebelow. Plot cell survival as a function of dose and use thelinear-quadratic model to fit the data.
d) Determine the alpha/beta ratio from the curve fit. Is this anearly responding or late responding cell line?
Number of cells plated | Number of colonies counted | Dose (Gy) |
100 | 75 | 0 |
400 | 255 | 2 |
400 | 180 | 5 |
1000 | 150 | 10 |
10000 | 90 | 20 |
