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Q3 In the presence of a template DNA, a primer and deoxyribonucleotides, DNA polymerase synthesises DNA...

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Biology

Q3 In the presence of a template DNA, a primer anddeoxyribonucleotides, DNA polymerase synthesises DNA by addingcomplementary bases at the end of the primer/growing chain. The DNAtemplate and the primer sequences are as follows DNA template 5`-ATGCGTGCGTACATACGCAATTCGAATGCGCGCTAACGGCCTTGCG-3` Primer5`-CGCAAGGCCGTTAGCGC-3`

a. Deduce the sequence of the next five bases that will be addedto the primer when all reagents needed for DNA synthesis aresupplied in the reaction.

b. Generate the sequence of the newly synthesised shortestlabelled fragment if labelled ddATP was also added in the reactionmixture?  

Answer & Explanation Solved by verified expert
4.0 Ratings (675 Votes)
a DNA template sequence is 5 ATGCGTGCGTACATACGCAATTCGAATGCGCGCTAACGGCCTTGCG3 Complementary Strand sequence is 3 TACGCACGCATGTATGCGTTAAGCTTACGCGCGATTGCCGGAACGC5 Primer sequence is 5CGCAAGGCCGTTAGCGC3 The sequence of the    See Answer
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