Quantification of DNA Using Agarose Gels
Directions: Please answer thefollowing questions in complete sentences.
What problems might arise if the agarose wasn't dissolved/meltedcompletely or if there were air bubbles in the gel?
Why did your DNA samples need to be mixed with a loading dyeprior to loading the samples into the gel? Give 2reasons.
What causes the DNA to travel through the gel?
Draw a sketch of what your gel would have looked like (after 5minutes) if you reversed the positive and negative leads and placedthe positive lead closest to the wells. Label thepositive and negative leads, the wells, and the DNA.
Why do smaller DNA fragments travel further down the gel?
