Question 1 a) What is the significance of the N.A. numberinscribed on the outer barrel of an objective? b) What is meant bydepth of field? c) With an objective of a given magnification, whyshouldn't one use increasingly higher magnification eyepieces toachieve higher total magnification? d) In a light microscope anobject is placed 2 mm away from a lens of diameter 2 mm. The objectis in air (refractive index = 1) and the wavelength of the light(green) is 520 nm. i. What is the one-half angular aperture of theobjective? ii. What is the best possible resolving power of thismicroscope? e) Calculate the eth o iel or a resolin oer o m in amicroscoe ith a final aperture of diameter 1 mm and a workingdistance of 20 mm. f) Why is optical microscopy not suitable forobservations of fracture surfaces? g) What is the advantages of theconfocal microscopy over optical microscopy?
