Question 1
Let’s start with a relatively straightforward one – and make ittopical.
- Briefly explain what qPCR is and why it is differentthan traditional PCR. Address what the q stands for and why thetechnique is sometimes called real-time pcr.
Unfortunately – that real-time moniker became a problem becausesome people shortened it to RT-PCR.However, most usage of RT-PCR issomething distinct from qPCR.
- Describe that something else RT-PCR.
You can combine qPCR and RT-PCR – producing RT-qPCR. Oneapplication of RT-qPCR is detection of certain viruses – such asCOVID-19.
- For this application, describe why RT-qPCR ispreferable to qPCR.
- For this application, describe why RT-qPCR ispreferable to RT-PCR.
