Question 2
It is known that some tumors are associated with tumor
viruses. It remains unknown, however,...
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Biology
Question 2
It is known that some tumors are associated with tumorviruses. It remains unknown, however, whether or not other tumorssuch as those induced by chemical carcinogens are also associatedwith tumor viruses.
Design a series of experiments to test the hypothesis thatnon-viral carcinogen-induced tumor cells contain a gene(s) that isaltered (as compared to its unaltered state in the normal cellcounterparts) and is responsible for the transformed phenotypes ofthe tumor cells.
Include in your experimental design the following:
1) the overall strategy,
2) the choices of reagents including controls and therationales behind each of the choices,
3) at least four types of transformation assays, includingboth in vitro and in vivo assays, to be used to measure theproperties of transformed cells, and
4) the strategy to interpret the results and to draw aconclusion.
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Let us take the effect of chronic exposure of aflatoxin B1 from Aspergillus spore on liver cancer It is known that aflatoxin induces p53 a tumour suppressor mutations leading to abrogation of its activit and initiation followed by progression of the tumour To detect whether the carcinogen has caused mutation in say p53 one can obtain p53 from normal liver cell line AML12 and obtain the p53 gene after cDNA
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