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Question 30: A. Describe the secondary structural characteristics of B-form double-stranded DNA. B. Explain how to determine the...

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Biology

Question 30:

A. Describe the secondary structural characteristics of B-formdouble-stranded DNA.

B. Explain how to determine the number of supercoils present ina molecule of DNA.

C. Explain/demonstrate how to determine changes in linkingnumber for DNA.

Answer & Explanation Solved by verified expert
3.9 Ratings (383 Votes)
30 A DNAs secondary structure is predominantly determined by basepairing of the two polynucleotide strands wrapped around each other to form a double helix Bform DNA The information from the base composition of DNA the knowledge of dinucleotide structure and the insight that the Xray crystallography suggested a helical periodicity were combined by Watson and Crick in 1953 in their proposed model for a double helical structure for DNA They proposed two strands of DNA each in a righthand helix wound around the same axis The two strands are held together by Hbonding between the bases in anti conformation Bases fit in the double helical model if pyrimidine on one strand is always paired with purine on the other From Chargaffs rules the two strands will pair A with T and G with C This pairs a keto base with an amino base a purine with a pyrimidine Two Hbonds can form between A and T and three can form between G and C This third Hbond in    See Answer
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