Question 5 (alternate). Gene and protein expression (Total 4marks)
A. You have successfully completed your two-channel microarrayexperiment and have obtained Red and Green intensity values foreach feature/probe on the array.
i. Describe how you would initially transform the data so thatincreases and decreases in gene expression are treated equally. (1mark)
ii. What method would you use to normalise the intensities ofthe two dyes, as well as to eliminate other systematic differencesdue to unequal experimental conditions. (1 mark)
B. You wish to normalise data values between arrays. Providedetails of a method for normalising data between arrays.