Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. rnai is used to functionally inactivate genes in c

RNAi is used to functionally inactivate genes in cells and whole organisms like C. elegans. Describe...

80.2K

Verified Solution

Question

Biology

RNAi is used to functionally inactivate genes in cells and wholeorganisms like C. elegans. Describe the basics of how you wouldknock down the expression of a gene required for muscle formationin C. elegans and what method could you use to confirm that yourresults were specifically attributed to the RNAi?

Answer & Explanation Solved by verified expert
3.9 Ratings (747 Votes)
RNA interference RNAi is a commonly used technique for reverse genetic approaches in Caenorhabditis elegans Feeding RNAi is the most convenient and inexpensive method for performing genomewide RNAi screens In Caenorhabditis elegans many of the critical proteins involved in this process have been uncovered through mutational screens focusing on uncoordinated movement and embryonic arrest phenotypes Caenorhabditis elegans is a valuable model organism for the study of muscle due to the similarity of worm body wall muscle to vertebrate muscle along with its semitransparent cuticle that allows for visualization of muscle structures in vivo The basic protein components within a C elegans sarcomere have vertebrate counterparts with only minor differences in protein composition and organization MATERIALS AND METHODS Acquiring C elegans strains Wildtype C elegans strains Preparing E coli OP50    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

Ch. 3: Molecular diversity Relate the structure of each macromolecule with its function Contrast the structure of DNA...

Biology
Q

Class, what is the primary purpose of investing in Stock Index Futures? Name any 3 advantages of...

Finance
Q

Describe why hypothesis testing is important to businesses?

Basic Math
Q

A solid non conducting sphere of radius R having variable volume charge density p where...

Physics
Q

2 Determine if each of the following is a group a monoid that is not...

Basic Math
Q

Find the probability that of 25 randomly selected students, at least two share the same...

Basic Math
Q

The distribution of actual weights of 8 ounce wedges of cheddar cheese produced at a...

Statistics
Q

Botosan Factory has budgeted factory overhead for the year at $821,800, and budgeted direct labor...

Accounting
Q

------------ Preble Company manufactures one product. Its variable manufacturing overhead is applied to production based...

Accounting
Q

***Please show how to get answer*** 38. Of the following ownership rights,...

Accounting
Q

Prepare flexible budget report; compare flexible and static budgets. E10.8 (LO 1, 2), E Service...

Accounting
Q

You have a 30-year mortgage with 6% annual rate and the principal amount of $250,000....

Accounting