Set IHMR
A | Relative Concentration
Methyl Red | Absorbance (a.u.)
at 425 nm |
|---|
| A0 | 1.00 | 0.094 |
| A1 | 0.75 | 0.058 |
| A2 | 0.50 | 0.041 |
| A3 | 0.25 | 0.030 |
Set IIHMR
A | Relative Concentration
Methyl Red | Absorbance (a.u.)
at 520 nm |
|---|
| A0 | 1.00 | 0.895 |
| A1 | 0.75 | 0.806 |
| A2 | 0.50 | 0.597 |
| A3 | 0.25 | 0.224 |
Set IMR-
B | Relative Concentration
Methyl Red | Absorbance (a.u.)
at 425 nm |
|---|
| B0 | 1.00 | 0.480 |
| B1 | 0.75 | 0.302 |
| B2 | 0.50 | 0.243 |
| B3 | 0.25 | 0.052 |
Set IIMR-
B | Relative Concentration
Methyl Red | Absorbance (a.u.)
at 520 nm |
|---|
| B0 | 1.00 | 0.048 |
| B1 | 0.75 | 0.033 |
| B2 | 0.50 | 0.023 |
| B3 | 0.25 | 0.017 |
Based on the data above, calculate the slope of the line of theabsorbance vs relative concentration for each for the four datasets (Part III in your lab manual).
Let:
αHMR
IA equal the slope for solutions A0-A3 at 425mm
αMR-
IB equal the slope for solutions B0-B3 at 425mm
αHMR
IIA equal the slope for solutions A0-A3 at520 mm
αMR-
IIB equal the slope for solutions B0-B3 at520 mm
These α's are the respective Beer's Law constants for HMR andMR- at the 2 wavelengths. Enter below the α's that youdetermined from your Excel graphs
