Show all calculations in a neat and organized manner. Be surethat I understand your logic. For your experiment you must treat 1X 109 cells with 5 nM cycloheximide (MW=281.35). Cycloheximide isboth toxic and expensive. You have counted the cells with ahemocytometer and found the following numbers when counting 5 “4x4â€sections (each 4x4 is 0.004 µl) of a 1:10 dilution of you chlamystock: 90, 103, 123, 99, 107.
1) What is the concentration of the initial Chlamy culture incell/ml? _______________________
2) How many mls of cells of the culture must you use for theexperiment? _____________________
3) Describe how you will treat the cells with cyclohexamide.Clearly show all calculations.
