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Sketch 2 curves on the same graph for mammalian cells: after X-rays and after alpha-particles irradiation....

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Biology

Sketch 2 curves on the same graph for mammalian cells: afterX-rays and after alpha-particles irradiation. Explainquantitatively the dependence on radiation dose of the survival forX-ray curve at low doses (up to 0.5Gy), at very high doses (higherthan 7Gy). Discuss the mechanisms involved in cell death.

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A cell survival curve is a plot of the number of cells that survive to form colonies as a function of radiation dose Thus cell survival curves measure reproductive cell death Some damaged cells may continue to function for a time but if they do not reproduce they are not counted as survivors In a cell survival experiment cells are seeded onto petri dishes and exposed to various doses of radiation The number of cell colonies that subsequently grow is determined Each colony is assumed to be derived from a single surviving cell A cell survival curve is a plot of the fraction of cells that survive normalized by the fraction of cells that survive with no radiation exposure versus the radiation dose each point on a cell survival curve corresponds to a single dose of radiation Usually the log of the surviving fraction is plotted on the vertical axis versus dose on the horizontal axis Mammalian cell survival curves generally show an exponential response to high doses of radiation with a shoulder of varying widths in the lowdose range The effect of radiation on cells can be observed by noting how the cell survival curve changes under different conditions These changes in condition include using different    See Answer
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