Steps
a) add 20 grams of oysters to 20 mL of saline to give a 1:2dilution slurry
b) Transfer 5 grams of the slurry into a conical tube.
c) Prepare a 1:10 serial dilution by adding 20mL of saline tothe measured 5 g of slurry ( this step effects a 1:5 dilution butthat sample was already diluted 1:2)
d) Prepare a 10^-1, 10^-2, and 10^-3 serial dilution in culturetubes with 9 mL of saline
e) plate 0.1 ml of each dilution onto an agar plate.
f) two plates from the 10^-3 dilution made colonies of 112colonies and 58 colonies.
1. Show calculations for the serial dilutions with units
2. calculate the CFU/g for each plate with colonies withunits
