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Substrate	Treatment __Sucrose________			Treatment ___NaCl/Sucrose__			Treatment __EtOH__________			Treatment			Treatment ___EtOH/Sucrose__
										__Na saccharin____
Time	10%	5%	2.50%	10%	5%	2.50%	10%	5%	2.50%	10%	5%	2.50%	10%	5%	2.50%
(minutes)
0	0.995	0.978	0.755	1.172	1.137	1.095	1.065	1.018	1.018	1.078	1.053	1.050	0.926	0.974	1.010
30	0.959	1.001	0.970	1.010	1.007	1.073	1.124	1.038	1.050	1.032	1.058	1.047	0.928	0.961	1.039
60	0.856	0.909	0.915	1.073	1.041	1.037	1.040	1.018	0.878	1.017	0.991	1.038	0.947	0.971	1.080
90	0.909	0.847	0.887	1.028	1.091	1.132	0.976	0.961	0.881	1.027	1.026	1.037	0.936	0.993	1.071
120	0.953	0.933	0.985	1.035	1.056	1.087	1.018	1.081	1.102	0.868	0.855	0.853	0.838	0.899	0.995
150	0.834	0.897	0.949	1.059	1.080	1.110	0.964	1.130	1.107	0.997	1.031	1.015	0.957	1.016	1.162
180	1.010	0.941	0.969	1.029	1.107	1.119	0.677	1.130	1.122	0.970	1.011	1.028	0.987	1.061	1.249
210	0.958	0.996	1.015	1.060	1.069	1.226	0.762	1.157	1.185	0.994	1.041	1.052	1.021	1.099	1.280
240	1.009	1.065	0.972	1.069	1.080	1.211	0.735	1.171	1.187	0.985	1.032	1.028	1.002	1.146	1.309
270	1.050	1.009	1.186	1.040	1.082	1.169	0.761	1.261	1.144	0.976	1.068	1.094	1.057	1.183	1.351
300	1.098	1.056	1.230	1.058	1.074	1.174	0.838	1.219	1.135	0.981	1.085	1.132	1.089	1.215	1.393

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1) Calculate growth rates. This has been done for you - see dataset supplied on Moodle. 2) Create 5-line charts using Excel or Google Sheets (See Tutorials on Moodle). Plot data from table 3 (Growth vs. time). The three different concentrations for each treatment should be represented on a single graph. Make sure you create a legend, which identifies the three concentrations (10%, 5%, 2.5%). Questions - answer as a part of your assignment. 1) Explain the processes of glycolysis and fermentation. What are the main differences between these two processes? 2) Which concentration is best for each of your substrates? 3) What is the best substrate for growth of yeast? Justify your answer. 4) What is the worst substrate for growth of yeast? Justify your answer. 1) Calculate growth rates. This has been done for you - see dataset supplied on Moodle. 2) Create 5-line charts using Excel or Google Sheets (See Tutorials on Moodle). Plot data from table 3 (Growth vs. time). The three different concentrations for each treatment should be represented on a single graph. Make sure you create a legend, which identifies the three concentrations (10%, 5%, 2.5%). Questions - answer as a part of your assignment. 1) Explain the processes of glycolysis and fermentation. What are the main differences between these two processes? 2) Which concentration is best for each of your substrates? 3) What is the best substrate for growth of yeast? Justify your answer. 4) What is the worst substrate for growth of yeast? Justify your

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