Suppose that you are given the task of determining theSCN- content of a blood sample by forming theFe(SCN)2+ complex and measuring the absorbance. Youbegin by taking a 10.00-mL volume of the blood sample, centrifugingit, transferring 0.50 mL of the clear serum to a flask, and thenadding 15.00 mL of 0.20M Fe3+. The %T of this solution,measured at 460 nm in a 1.0-cm diameter cuvet, is 75%. Acalibration curve, prepared from Fe(SCN)2+ standards,indicates that the Fe(SCN)2+ concentration of thediluted sample solution is 0.033 mM. Calculate the SCN-content (in mM) of the original blood sample.
(Atomic weight of Fe = 55.85 g/m; formula weight ofSCN- = 58.08 g/m)
A) 0.033mMÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â
B) 0.33mMÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â
C)0.40 mM
D) 0.82mMÂ Â Â Â Â Â Â Â Â
E) 1.02 mM
