The absorbance of a 1 mg/ml sample of lysozyme was measured in acuvette of thickness 1.00 cm and was found to be 3.19 at 240 nm and2.58 at 280 nm. The molar absorption coefficients of tryptophan andtyrosine at 240 nm are 2.00 x 103 dm3 mol-1cm-1 and 1.12 x 104 dm3 mol-1cm-1, respectively, and at 280 nm they are 5.40 x 103dm3 mol-1 cm-1 and 1.50 x 103 dm3mol-1 cm-1, respectively. What are the molarconcentrations of the two amino acids in the 1 mg/ml proteinsample? If the molar mass of the protein is 14,400 g/mol, how manytryptophans and tyrosines are present in the sequence of thisprotein? (looking for the molar ratio, moles aa/mol protein) Whatis the molar absorption coefficient of the lysozyme protein at 280nm? What would be the % Transmittance of the same protein sample ifmeasurements were made in a 0.1 cm cuvette at 280 nm?
