The amino acid leucine has two titratable groups, the α-amino,pKa 9.6 and the α-carboxyl, pKa 2.4. Calculate the ratio of acidicand basic forms and express them as [A]/[HA] ratio or [B]/[BH] ofthe titrating group at the following pH values: α-carboxyl @ 1.4,1.9, 2.4, 2.9, 3.4; α-amino @ 8.1, 8.6, 9.1, 9.6, 10.1. Plot pH vs.[B]/[BH] for each group, on the same graph. Compare similarities ordisparities in the titration of each group.
