The bacterial one-hybrid assay holds E. coli cellshostage until our protein of interest can active transcription andtranslation of enzymes from the HIS3 and URA3 genes. These enzymesare needed to produce Histidine and Uridine, which are both neededfor cells to grow and divide. Make an estimate, based on enzymekinetics and any other information you may need, of how many HIS3and URA3 enzymes will an E. coli cell need to grow anddivide at a normal rate. How many are needed when we add 10 mM3-amino-1,2,4-triazole (3AT) to the growth medium as a competitiveinhibitor of HIS3?
