The data to the below were collected for the bovine carbonicanhydrase-catalyzed reaction described by
CO2 (aq) + H2O (l) -->H+ (aq) + HCO3- (aq)
[CO2] (mmol * L^-1) | R (õmol * L^-1 * s^-1) |
1.25 | 28.74 |
2.50 | 48.61 |
5.00 | 80.28 |
20.00 | 155.59 |
Use these values to determine the value of KM, theMichaelis-Menten constant. Answer the four parts below.
a) If you were to plot this data to graphically determine Rmaxand KM using a Lineweaver-Burk plot, what would you plot for thefollowing data? If you would plot the original data, enter theoriginal value in the corresponding blank.
[CO2] (mmol * L^-1) graph as R (õmol * L^-1 * s^-1) graphas
1.25--> ________ 28.74 --> ________
20.00--> ________ 155.59 --> ________
b) Calculate the slope using the data points from part (a). Thendetermine the y-intercept using the second point.
Slope= _______ s y-intercept= ___________ L * s * mmol^-1
c) Use the y-intercept to calculate Rmax.
Rmax=___________ mmol * L^-1 * s^-1
d) Use Rmax and the slope to calculate Km.
Km=___________ mmol * L^-1
