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The enzyme is studied in the presence of a different inhibitor (inhibitor B). In this case,...

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Chemistry

The enzyme is studied in the presence of a different inhibitor(inhibitor B). In this case, two different concentrations ofinhibitor are used. Data are as follows:

a) Determine the apparent Vmax at each inhibitorconcentration.

b) Determine the apparent KM at each inhibitorconcentration.

c) Estimate KI from these data. [Hint: Calculate theKI at 3 mM and at 5 mM; and then take the average of thetwo KI values.]

v[(mmol/L)min−1]
[S](mmol/L)No inhibitor3 mM inhibitor B5 mM inhibitor B
1.251.721.251.01
1.672.041.541.26
2.502.632.001.72
5.003.332.862.56
10.004.173.703.49

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