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The extinction coefficients of the four natural nucleoside-5’monophosphates pdA, pdG, pdC and pdT at 260 nm...

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Chemistry

The extinction coefficients of the four naturalnucleoside-5’monophosphates pdA, pdG, pdC and pdT at 260 nm are1.506*104 , 1.218*104 , 7.10*103 ,and 8.56*103 L/mol/cm, respectively (Cavaluzzi, M.J.,Nucleic Acids Res., 2004, 32, e13). Please determine:

a) The extinction coefficient of a single-stranded,5’-phosphorylated Dickerson Dodecamer sequenced(pCGCGAATTCGCG).

Answer: ε260 = 1.244*105. HOw they got that?

b) Please calculate absorbance and percent transmittance of a 10micromolar (μM) solution of the single-stranded DickersonDodecamer, as described in (a), at 260 nm when a cell with the pathlength of 1.0 cm was used.

What is A? %T?

I don't know the answer for that.

Answer & Explanation Solved by verified expert
4.3 Ratings (656 Votes)
You have already mentioned the LambertBeer law For the solution of a single compound S in a cuvette of width d the absorbance E at a particular wavelength is proportional to the concentration c of S E io icd A higher concentration of S will result in higher absorbance Now imagine that the    See Answer
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