The following chart show the results of cloning nuclei fromseveral cell lines (e.g. v6.5, v18.6, etc.) derived from twodifferent genetic strains of mice (F1 and 129). Note: your answersrequire no prior knowledge of these strains or the specific celllines listed)
Genetic Background | Cell Line | Passage Number | Activated Oocytes | Embryos Transferred (% Activated) | Dead (% Transferred) | Alive (% Transferred) | Survived Long Term (% Transferred) |
F1 | V6.5 | 5-6 | 149 | 21 (14%) | 0 | 4 | 4 (19%) |
F1 | V6.5 sc84 | 8-9 | 46 | 6 (13%) | 2 | 2 | 2 (33%) |
F1 | rtA2 SD-18 | 8-9 | 32 | 7 (22%) | 0 | 1 | 1 (14%) |
Total (F1) | | | 227 | 34 (15%) | 2 | 7 (21%) | 7 (21%) |
| | | | | | | |
129 | V18.6 | 5-6 | 107 | 19 (19%) | 1 | 4 | 0 |
129 | J1 | 11 | 145 | 26 (18%) | 2 | 4 | 0 |
129high | J1 | 35 | 92 | 23 (25%) | 10 | 0 | 0 |
129 | LJG-13 | 8-9 | 74 | 8 (11%) | 4 | 0 | 0 |
Total (129) | | | 418 | 76 (18%) | 17 | 8 (11%) | 0 (0%) |
- What is the main conclusion that can be drawnbased on the results of these data with respect to success of thecloning experiments?
- Give three possible cellintrinsic differences between v6.5 cells andLJG-13 cells that could play a role in their different results inthis assay.
For one of the three potential factors you mention above,describe a brief experimental plan to test whetherthis factor is responsible for the differences between v6.5 cellsand LJG-13 cells in this assay. Include in this plan roughly howyou would perform the experiment, what your readout would be andwhat you could conclude based on your results.
