The following is a portion of the non-template DNA sequence fromthe human ras gene. Answer the following questions aboutthis gene and the effects of gene changes to the specified mutantsequence (mutation in BOLD).
Normal human ras:5’-acatgactgaatataaacttgtggtagttggagctggt-3’
Mutant human ras:5’-acatgactgaacataaacttgtggtagttggagctggt-3’
a. (4) What is the normal human Ras amino acid sequence fromthis portion of the gene, starting with the first start codon?
b. (2) Which amino acid from the normal sequence is mutated, andwhat is the amino acid changed to in the mutant sequence?
c. (3) What type of mutation is present in this gene (nonsense,missense, silent, transition, transversion)? Include all of thelisted terms that apply.
d. (1) Is ras a proto-oncogene or a tumor suppressorgene?
e. (3) Describe the normal cellular function of Ras. How might amutation in ras promote cancer formation, based on thisnormal cellular function described in your textbook?
