- The molecular weight of anhydrous calcium chloride is 110.99grams/mol. How many grams do you need for 750 ml of 250uM calciumchloride?
2. Ethanol is supplied in a 90% solution. You need 275 mL of 75%Ethanol for your experiment. How would you make this solution?
3.You have 12 mL of cells you need to treat with HydrogenPeroxide (H2O2), such that the finalconcentration of H2O2 is 50 uM. How much of a30 mM stock solution of H2O2 would you add toyour cells?
4. You need to prepare a stock solution of medium for yourculture cells, which usually includes liquid salt solution andbovine serum. Your liquid salt solution is supplied in a 50Xconcentration, and you need to dilute it to 1X for use. You alsoneed to add 75% fetal bovine serum for a final concentration of15%. How would you make up 0.80 liters of this culture media usingwater as your solvent?
5. You need to treat cells with arsenite such that it has afinal concentration of 25 uM once it has been added to the cells.You need to treat 10mL of cells, and you don't want your treatmentvolume to be more than 7 uL per 1mL of cells. What concentrationshould you make your stock solution?
