The ubiquitin protein from the malaria parasite was treated with
chymotrypsin, and the resulting fragments were...
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Biology
The ubiquitin protein from the malaria parasite was treated withchymotrypsin, and the resulting fragments were sequenced. A secondsample of the polypeptide was treated with trypsin, and thefragments were sequenced. What is the sequence of thepolypeptide?
Chymotrypsin fragments:
AGKQLEDGRTLSDY
IPPDQQRLIF
VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI
NIQKESTLHLVLRLRGGMQIF
Trypsin Fragments:
LR
AK
EGI
IQDK
LIFAGK
QLEDGR
TLTGK
IPPDQQR
GGMQIFVK
TLSDYNIQK
ESTLHLVLR
Answer & Explanation
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3.9 Ratings (760 Votes)
The sequence is IPPDQQRLIF AGKQLEDGRTLSDY NIQKESTLHLVLRLRGGMQIF VKTLTGKTITLDVEPSDTEINVKAKIQDKEGI Chymotrypsin cleavage site is NHRRCO R phenylalanine F Tyrosine Y tryptophan W R is not proline P The cleavage site of trypsin is NHRCO R Lysine K or arginine R In both
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