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This is for my biochemistry lab, the experiment is dealing with trypsin and BPTI. I need...

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Chemistry

This is for my biochemistry lab, the experiment is dealing withtrypsin and BPTI. I need to make a graph: plot the absorbancechange per minute versus the BPTI concentration for eachcuvette.

here are my cuvettes and amount of BPTI added to each

1- 0uL BPTI added

2- 10 uL BPTI added

3-20 uL BPTI added

4-30 uL BPTI added

5- 40 uL BPTI added

6- 50 uL BPTI added.

Each cuvette has a different amount of water and trypsin addedto them, for a total volume of 100 uL in each cuvette.

I was give a sample of BPTI for which I had to find theconcentration. Using the absorbance, I calculated that theconcentration was 0.035mM. We then had to dilute this 10-fold, soto 0.0035mM.

I am not sure how to find the concentration of BPTI in eachcuvette with this information. The molecular weight of BPTI is6500. I don't know if that is needed.

I feel like this should be really easy, but I am having troubledoing this.

Answer & Explanation Solved by verified expert
3.9 Ratings (475 Votes)
Make a table SNo BPTI uL Water Trypsin OD 1 0 90 10 2 10 80 10 3 20 70 10 4 30 60 10 5 40 50 10 6 50 40 10 BPTI    See Answer
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