To make 10X gel electrophoresis running buffer, 30.0 g Tris
base, 144.0 g glycine, and 10.0...
80.2K
Verified Solution
Link Copied!
Question
Chemistry
To make 10X gel electrophoresis running buffer, 30.0 g Trisbase, 144.0 g glycine, and 10.0 g SDS must be dissolved into 1000mL water. The working stock is a 1X concentration. a. What is theconcentration in % w/v of each of the 3 reagents in the 10X stock?b. Describe how you would make 1 L of a 1X working stock solution.c. If 50 mL of the 1X working stock are used to run a gel, how manygrams of each of the 3 reagents are in the 1X solution?
Answer & Explanation
Solved by verified expert
4.3 Ratings (700 Votes)
a Volume of water 1000 ml Mass of water volumedensity 100010 1000 g Total mass of solution mass of watermass of trismass of glycinemass of SDS 10003001440100 11840 g Considering density as 10 gml of solution volume
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
