Treatment of cells with the BOBA drug induces apoptosis of cellsand leads to DNA fragmentation.
A) (4pts) Describe the cellular mechanism that causes DNAfragmentation during apoptosis to occur. (4 sentence max)
B) (8pts) Describe an experiment, step by step, that can be used todetermine how many hours after BOBA treatment DNA fragmentationoccurs. (8 sentence max)
C) (4pts) One of your friends said they would use an Annexin V andPI staining technique to identify how many hours post BOBAtreatment DNA fragmentation occurs. Why won’t this experimentalmethod work to answer this experimental question? (4 sentencemax)
