Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for
studying biochemical processes, is prepared by...
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Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer forstudying biochemical processes, is prepared by a biological chemistusing 10.0M NaOH to a pH of 7.79 using 31.52 g of (HOCH2)3CNH3Cl,or TrisH+, (MW=157.597g/mol, pKa 8.075).
(a) Calculate the ratio of [Tris]/[TrisH+] at a pH of7.79.
(b) Calculate the initial moles of (HOCH2)3CNH3Cl(MW=157.597g/mol) that were added.
(c) Write the chemical reaction between the TrisH+ and theadded NaOH.
(d) Calculate the moles of hydroxide used to produce the ratiocalculated in part (a).
(e) What volume of 10.0 M NaOH is needed to prepare thebuffer?
(f) Now you dilute the solution from above to 1.0 L and takehalf of the buffer (500.0 mL) and add 0.0100 moles solid HCl. Whatis the new pH?
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