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Use the Beer-Lambert law to explain how a colorimetric assay works in the spectrophotometer. In your...

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Biology

  1. Use the Beer-Lambert law to explain how a colorimetric assayworks in the spectrophotometer. In your answer, define thefollowing terms; chromophore, extinction coefficient, absorbance,transmission and reagent blank.

  1. Most of the spectrophotometers in the teaching lab emit lightof wavelengths between 400-700nm. However some spectrophotometersalso emit light between 200-400nm. Why is this extended rangeuseful? Provide a specific example.

  1. How can you use a spectrophotometer to perform a qualitativeanalysis of a substance?

Answer & Explanation Solved by verified expert
4.4 Ratings (690 Votes)
1 The procedure based on absorption of visible light is called colorimetry The colorimeter is based on BeerLamberts law According to the law the absorption of light transmitted through the medium is directly proportional to the medium concentration A beam of light with a specific wavelength is passed through a solution via a series of lenses in a colorimeter The colored light is navigated to the measuring device This analyzes the color compared to an existing standard A microprocessor then measures the absorbance or percent transmittance Higher    See Answer
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