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Using DNase footprinting, show that the sigma subunit of prokaryotic RNA Pol binds to nucleotides from...

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Biology

Using DNase footprinting, show that the sigma subunit ofprokaryotic RNA Pol binds to nucleotides from -35 to -10 in thepromoter region.

a. Outline your experiment and draw and label the data. Explainhow your data support this conclusion.   

b. Show and label the data for a similar experiment done usingDMS footprinting rather than DNase foot printing. Explain how yourdata support this conclusion.  You do NOT have to writeout the experiment again.

Answer & Explanation Solved by verified expert
4.1 Ratings (642 Votes)
DNA footprinting is a technique for exploring the arrangement specificity of DNArestricting proteins in vitro This procedure can be utilized to contemplate proteinDNA communications both outside and inside cells A DNase footprinting test is a DNA footprinting strategy from atomic sciencenatural chemistry that distinguishes DNAprotein communication utilizing the way that a protein bound to DNA will frequently shield that DNA from enzymatic cleavage This makes it conceivable to find a protein restricting site on a specific DNA particle The technique utilizes a protein deoxyribonuclease DNase for short to cut the radioactively endmarked DNA trailed by gel    See Answer
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