Using the blanks provided, put the steps of involved in mappinga bacterial chromosome by conjugation in chronological order usingthe numbers 1-6 in the blanks provided.
a. ___ ___ Cells that mated are checked for transfer of othergenes from the Hfr to the F– strain.
b. ___ ___ At regular intervals, conjugation is interrupted byshearing the mating bacteria away from each other.
c. ___ ___ For each time point, cells that mated areselected—for instance, by requiring that a prototrophy orantibiotic resistance from each parent be present in one cell.
d. ___ ___ Select a donor Hfr strain and a recipient F– strain.The two strains must be different in genotype for several markergenes—for example, leu– and leu+, azir and azs.
e. ___ ___ The two strains are mixed together in a nutrientmedium to allow conjugation to begin. After a few minutes, theculture is diluted to prevent new conjugations.
f. ___ ___ Order and distance of transferred genes aredetermined by comparing the time required for them to transfer fromthe Hfr to the F– strain.
