Using the
Michaelis-Menten equation, derive an expression that will
determine Km
as a function or in
terms of Vmax,
V0
and...
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Chemistry
Using theMichaelis-Menten equation, derive an expression that willdetermineKmas a function or interms ofVmax,V0and [S]. With thisderived equation then calculate:
a)Km
B) Indicate ateach substrate concnetration whether this Km changes with thechanging S
C)Using the doublereciprocal plot, determineKm.
Answer & Explanation
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4.1 Ratings (566 Votes)
1The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction Vmax The relationship between rate of reaction and concentration of substrate depends on the affinity of the enzyme for its substrate This is usually
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