We will assay enzyme activity at 340 nm. The molar absorbance is
higher at 260, meaning...
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We will assay enzyme activity at 340 nm. The molar absorbance ishigher at 260, meaning we can detect three times less cofactor. Whydon’t we use that wavelength? If you had to pick the worst possiblewavelength to monitor, what would it be more context: doing assaysafter purifying proteins from animal tissue. Testing for enzymaticactivity
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One of the methods of testing or determining enzymatic activity is spectrophotometric enzyme assays During a spectrophotometric enzyme assay an operator follows the course of an enzyme reaction by measuring the changes in the intensity of the light absorbed or scattered by the reaction solution If an enzyme
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