What are the components of a PCR reaction? How would you
calculate the final concentrations of...
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Biology
What are the components of a PCR reaction? How would youcalculate the final concentrations of the components in the PCRreaction and in the restriction digest? What happens during eachstep of a PCR cycle? What parameters vary amount restrictionenzymes?
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PCR stands for Polymerase Chain Reaction it is a molecular technique that is used to amplify the target gene sequence Components of PCR the chemical components invovled in the reaction tube are DNA template taq DNA polymerase enzyme primers dNTPs deoxy nucleoetides like dATPs dCTPs dTTPs dGTPs reaction buffer and Mgcl2 DNA template is the target DNA molecule taq DNA ploymerase is the enzyme that is used to amplify the DNA invitro and it works at higher temperatures primers
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