What are the primary differences between a laser scanning
confocal and a spinning disk confocal microscope?...
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Biology
What are the primary differences between a laser scanningconfocal and a spinning disk confocal microscope? Consider
(a) the physical design of the microscope
(b) the method of forming an image
(c) the detector that is used to capture the image.
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a Laser scanning confocal1Physical design of the microscopeIn laser scanning confocal systems point laser excitation andthe emission pinhole combine to remove most outoffocus lightThe emission pinhole size is adjustable2 Laser scanning confocal microscopes offer advantagesparticularly in imaging thick cells with a high density ofintracellular components These instruments allow extremely thinoptical sectioning of specimens enabling structures andintracellular features deep within the specimen to be
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