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What is meant by a “house-keeping” gene? Why is performing PCR on these an important control...

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Biology

  1. What is meant by a “house-keeping” gene? Why is performing PCRon these an important control in qPCR?

  1. Will the relative transcript levels of a gene always be thesame as corresponding protein levels? Discuss why or why not.

  1. What is meant by real-time PCR? In your answer, explain theterms cycle threshold and SYBR green.

Answer & Explanation Solved by verified expert
4.2 Ratings (876 Votes)
Housekeeping genes are also known as Constitutive genes as these genes are constitutively expressed in all cells in normal as well as diseased condition The genes are transcribed at a constant level and are required for carrying out basic functions by the cell Now these genes are an important control used while performing qPCR Quantitative PCR This particular PCR assay measures the amplification or is for quantification As housekeeping genes are constitutively transcribed at a constant level and are considered to be ideal can be best served as the control when quantifying other gene products This is also called Normalization whose purpose is to remove the nonbiological variation as much as possible and as HK genes remain stable    See Answer
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