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What is the best immunological assay for each of the following situations? For each situation, name...

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Biology

What is the best immunological assay for each of the followingsituations? For each situation, name the assay, describe how itworks, and why it is appropriate. Assume in both cases that anytype of specific antibody needed for the assay you propose isavailable.

a. Determine the number of memory T cells and naïve T cellsbased on the level of CD45RA present on the surface of the Tcells.

b. Determine the level of IgG specific to measles in the bloodof a person who has not been immunized but may have beenexposed.

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1In the peripheral blood of patients with tuberculosis a novel human effectormemory CD4 T cell subset with a nonclassical naivelike T cell phenotypes is identified These cells were CD45RO CD45RA CCR7 CD62L CD27 and capable of rapidly secreting multiple cytokines IFN TNF IL2 in response to different M tuberculosisantigens We have designated this CD4T cell population as TCNP cells T cells that are able to produce cytokines with a naive phenotype TCNP cells were further phenotyped as CD95lo CD28int CD49dhiCXCR3hi and a sizeable fraction ranging from 50 to 80 also expressed CD31    See Answer
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