What is the split gal4 system? Why is it needed to label some
cell types? Describe...
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Biology
What is the split gal4 system? Why is it needed to label somecell types? Describe an experiment that combines split gal4 withscRNA-seq to precisely define cell identities in the drosophilabrain. Describe the protocol of the experiment in detail
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The GAL4UAS system is a biochemical methodused to study gene expression and function in organisms such as thefruit flyThe Gal 4 system allows separation ofthe problems of defining which cells express a gene or protein andwhat the experimenter wants to do with thisknowledgeGal4 expression can be made even morespecific by means of intersectional strategies These can combinetwo different GAL4 linessay A and Bin a way that GAL4 is onlyexpressed in the cells that are in line A but not line B or thosethat are in both lines A and B When combined with intrinsicallysparse
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