What is Western blot used for? Describe how you would perform a
Western blot, starting from...
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Biology
What is Western blot used for? Describe how you would perform aWestern blot, starting from the sample preparation step to thefinal analysis.
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Western blot is often used to detect separate and identify specific protein molecules from among a mixture of proteins It is also called immunoblotting and is used in qualitative and semiquantitative analyses of proteins Steps in western blotting 1 Sample preparation First step in sample preparation is Lysis of cells Different methods are used to separate proteins from biological materials such as Mechanical disruption Liquid homogenization Sonication Freeze thaw and Grinding with liquid nitrogen Second step in sample preparation is Protein quantification This can be done in many ways as Absorbance method Biuret method Lowry
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