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  3. why, in an immunostaining procedure for a specific

why, in an immunostaining procedure for a specific antigen, do you normally use two antibodies sequentially?...

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Biology

why, in an immunostaining procedure for a specific antigen, doyou normally use two antibodies sequentially? Support your answerswith citations from the scientific literature

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Immunostaining procedure is an antibody basedmethod used to detect a specific protein or targetprotein in a sample as antibodies are highly specificantibody binds to a particular protein in the tissue sectionantigenantibody interaction is seen by using chromogenicdetection in it enzyme conjugated and antibody cleaves a substrateto produce a colored precipitate at the    See Answer
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