Why is a V vs S plot often inadequate for determining enzymekinetic constants, and how do various alternate plotting methodsallow these values to be determined more adequately? Give examplesof alternate methods of kinetic analysis and their utility.
The V vs S plot is inadequate because it makes the assumptionthat it’s only for a single subunit and substrate. The line on thegraph usually represents a line that best fits the data and are notcompletely accurate. This only measures free enzymes or unstableenzyme substrate complex. An alternate method
