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Why is a V vs S plot often inadequate for determining enzyme kinetic constants, and how...

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Biology

Why is a V vs S plot often inadequate for determining enzymekinetic constants, and how do various alternate plotting methodsallow these values to be determined more adequately? Give examplesof alternate methods of kinetic analysis and their utility.

The V vs S plot is inadequate because it makes the assumptionthat it’s only for a single subunit and substrate. The line on thegraph usually represents a line that best fits the data and are notcompletely accurate. This only measures free enzymes or unstableenzyme substrate complex. An alternate method

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Enzyme kinetics is the study of chemical reactions acrried outby enzymes and information about enzyme kinetics reveal thefavourability of an enzyme at specific conditions regulation ofthe activity of the enzyme its role in metabolic processes and thehow a drug or agonist can modify or inhibit the functioning of theenzyme Enzyme kinetics can be studied by plotting the amount ofsubstrate present S on the Xaxis since it is the independentvariable and the reaction rate Von the Yaxis which woulddepend on the amount of substrate available Such single substratereactions can be represented by the MichaelisMenten kinetic modelof singlesubstrate reaction wherein the increase in    See Answer
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