Why is it important to have sterile workspaces and equipment
when performing cell transformations and culturing?
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Biology
Why is it important to have sterile workspaces and equipmentwhen performing cell transformations and culturing?
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Importance of aseptic conditions sterile conditions in and around cell cultures Successful cell culture and cell transfermation depends heavily on keeping the cells free from contamination by microorganisms such as bacterial fungi and viruses Nonsterile supplies media and reagents airborne particles laden with microorganisms unclean incubators and dirty work surfaces are all sources of biological contamination Aseptic technique designed to provide a barrier between the microrganisms in the environment and the sterile cell culture depends upon a set of procedures to reduce the probability of contamination from these sources The elements of aseptic technique are a sterile work area good personal hygiene sterile reagents and media and sterile handling Manipulation of cultured cells Among the common manipulations carried out on culture
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