You are a graduate student studying an inducible transcriptionfactor called X. You make a cellular lysate and carry out a seriesof centrifugation steps at increasing speeds to localize X. Youfind that prior to induction, X is the supernatant following afourth, very high speed ultracentrifugation step; however, afterinduction it is located in the pellet after only one relatively lowspeed ultracentrifugation step. What might this tell you about howX is regulated? What other techniques might you carry out toconfirm your results?
