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You are a microbiology lab technician and you need to quantify the viable cell concentration in...

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Biology

You are a microbiology lab technician and you need to quantifythe viable cell concentration in your stock culture beforebeginning an experiment. Since you do not know the range, youperform a serial dilution over several orders of magnitude. You usethe pour plate method and plate 1 ml for each dilution level, induplicate. Give the most accurate value for the number of viablecells in the original stock culture, in CFU / ml. Just enter anumber, do not type the units!

Since you want the practice, you attempt a count for all plates,even though some are likely too numerous to count. Only use valuesthat give you the best accuracy. That means only use values thatare a \"countable number\" of colonies (see text section).

Dilution series colony counts:

1:10: 860, 745

1:100: 123, 114

1:1000: 11, 15

1:10,000: 2, 4

Answer & Explanation Solved by verified expert
3.7 Ratings (357 Votes)
Ans 1 Average original CFU in 110 dilution Average of Observed CFU per mL inoculum dilution factor in two plates 860 cell mL1 110 745 cell mL1 110 2 8025 cells mL 2 Average original CFU    See Answer
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