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You are a researcher working in a molecular laboratory. One day, you need to run a...

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Biology

You are a researcher working in a molecular laboratory. One day,you need to run a batch of PCRs. You assembled the reagentsincluding Taq polymerase, made the reaction mixtures, andran them in a thermocycler.

After the PCR program ran to completion, you performed a gelelectrophoresis to check the quality of the PCR product. To yourdismay, all reactions appeared to have failed! Nothing showed up onthe gel.

You reviewed the thermocycler’s log, which displayed thefollowing information about your recent PCR cycles:

Denaturation: 91°C for 1 minute

Annealing: 50 °C for 40 seconds

Elongation: 65 °C for 3 minutes

Can this temperature profile explain the PCR failure? Explain inyour own words (4 points)

Answer & Explanation Solved by verified expert
3.7 Ratings (466 Votes)
This is general method of PCR wherein step1 Duringthermocycling a 1530 second denaturation at 95C isrecommended2AnnealingThe annealing step is typically 1560 seconds    See Answer
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