You are asked by your new boss to prepare 750 ml of a 0.5M pH8.4 tris- (hydroxymethyl) amminomethane (Tris) buffer. The Trisbase is provided in the solid form (mol. wt. 121.1 g/mole, pKa8.1). You are also supplied with solutions of 0.5 M HCl and 0.5MNaOH. Explain how you would prepare this buffer.
