Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. you are planning to use pcr to amplify several reg

You are planning to use PCR to amplify several regions of a piece of DNA. The...

80.2K

Verified Solution

Question

Biology

You are planning to use PCR to amplify several regions of apiece of DNA. The sequence of your template DNA is provided belowalong with the sequences of all available primers. Determine whereeach of the primers bind and answer the following:

5' AGGGCCAAATGAGATGAGTCAAAAGCTGCCGATAACCGGATAG 3'

3' TCCCGGTTTACTCTACTCAGTTTTCGACGGCTATTGGCCTATC 5'

Primer 1: 5-TTGGCC 3

P2: 5-GTCAAA-3

p3: 5-AACCGG-3

p4: 5-CCGGTT-3

P1 can bind to the bottom strand?

P4 can bind to the molecule at only one location

Which primer pair would you use to amplify a double stranded pcrfragment of any size from this template

Which primer has the lowest melting point

Using the two selected primers and added all of the PCRcomponents to a test tube answer the following as the polymerasechain rxn proceeds

The concentration of dNTPs will increase,decrease, stay thesame, as the rxn proceeds

The concentration of Taq DNA polymerase will increase,decrease,stay the same, as the rxn proceeds

Answer & Explanation Solved by verified expert
4.2 Ratings (919 Votes)
5 AGGGCCAAATGAGATGAGTCAAAAGCTGCCGATAACCGGATAG 3 3 CCGGTT 5 primer1 will bind to the above strand 5GTCAAA 3 primer2 will bind to the below strand 3    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

Life moves more quickly with every generation. There is more technology to introduce you to more...

Finance
Q

XZYY, Inc. currently has an issue of bonds outstanding that will mature in 25 years. The...

Finance
Q

Name and describe 5 function of the liver

Medical Sciences
Q

Enter the letters corresponding to all the true statements. 1. At a certain location there is...

Physics
Q

A circular swimming pool has a diameter of 20 m the sides are 4 m...

Calculus
Q

eve gral given below Check your answer your answer by differentiation C

Calculus
Q

A Zookeeper has a walrus with a nutritional deficiency She wants to make sure the...

Algebra
Q

SkyChefs, Inc., prepares in-flight meals for a number of major airlines. One of the companys...

Accounting
Q

\table[[Balances on Farch 91,,,],[Direct materialn unad (in March),$32,200,$38,260,],[Direct labar used (in March),23,230,21,200,],[Grerhead spplied (Korch),13,200,12,200,],[Costs during...

Accounting
Q

Copyright terms are very long now compared to when copyrights were first used in the...

Accounting
Q

During Heaton Company's first two years of operations, it reported absorption costing net operating income...

Accounting
Q

Logo Inc, has two data services departments (the Systems Department and the Facilities Department) that...

Accounting